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. Author manuscript; available in PMC: 2021 Mar 1.
Published in final edited form as: FASEB J. 2020 Jan 30;34(3):4540–4556. doi: 10.1096/fj.201901879RR

Fig.7.

Fig.7.

Effect of chronic morphine on miR-378a-3p expression in Be(2)C cells and brain regions of morphine tolerant mice. A). Effect of morphine on miR-378a-3p expression in Be(2)C cells. Total RNAs from Be(2)C cells treated with morphine (3 μM for 48 hours) or without morphine (Control) (n = 6) were used in RT-qPCR to determine miR-378a-3p expression, as described in Materials and Methods. Two-tailed Student t-test was used. *: p < 0.05.

B). Effect of morphine on miR-378a-3p expression in brain regions of the morphine tolerant mice. Total RNAs from the prefrontal cortex (Pfc), striatum (Str), brainstem (BS) and thalamus (Tha) of morphine tolerant mice (implanted morphine pellet (75 mg) for 3 days)(n = 10), and control mice (implanted placebo pellet for 3 days) (n = 10) were used in RT-qPCR to determine miR-378a-3p expression. Two ANOVA with Bonferroni’s post hoc analysis was used. ****: p < 0.0001.