Figure 3. Increased placental HIF-1α induced by AT₁-AA is responsible for the excess accumulation of adenosine and increased expression of Cd73 and Adora2b in the placenta.

Pregnant mice were injected with purified IgG from normotensive pregnant women (NT-IgG) or PE patients (PE-IgG) on E13.5 and E14.5, and in vivo siRNA-mediated Hif-1α mRNA knockdown was performed. Samples were collected on E18.5.

(A) HIF-1α protein in the placentas of PE-IgG treated pregnant mice co-injected with control siRNA (Con-siRNA) or Hif-1a siRNA was detected by immunoblotting.

(B) Placental Cd73 mRNA levels were quantified by real-time RT-PCR. (n=5 or 6 mice per group, **P<0.01 vs NT-IgG, ^#P<0.05 vs PE-IgG+con-si)

(C) Placental Adora2b mRNA levels were quantified by real-time RT-PCR. (n=5 or 6 mice per group, **P<0.01 vs NT-IgG, ^#P<0.05 vs PE-IgG+con-si)

(D) Adenosine levels in mouse placentas were quantified by HPLC. (n=4 or 5 mice per group, **P<0.01 vs NT-IgG, ^##P<0.01 vs PE-IgG+con-si)