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. Author manuscript; available in PMC: 2021 Mar 1.
Published in final edited form as: FASEB J. 2020 Jan 12;34(3):4041–4054. doi: 10.1096/fj.201902583R

Figure 4. LIGHT signaling via its receptors contributes to excess placental adenosine production and elevated expression of Cd73 and Adora2b in the placenta.

Figure 4.

Saline or LIGHT with or without neutralizing antibodies against lymphotoxin β receptor (anti-LTβR) or herpes virus entry mediator (anti-HVEM) was injected into pregnant mice on E13.5 and E14.5. In vivo siRNA-mediated Hif-1α mRNA knock down was conducted. Samples were collected on E18.5.

(A) Adenosine levels in mouse placentas were determined by HPLC. (n=4–7 per group, **P<0.01 vs Saline, #P<0.05 vs LIGHT, ✝P<0.05 vs LIGHT+con-si)

(B) Placental Cd73 mRNA levels were quantified by real-time RT-PCR. (n=3–6 per group, **P<0.05 vs Saline, #P<0.05 vs LIGHT, ✝P<0.05 vs LIGHT+con-si)

(C) Placental Adora2b mRNA levels were quantified by real-time RT-PCR. (n=3–6 per group, **P<0.01 vs Saline, #P<0.05 vs LIGHT, ✝✝P<0.01 vs LIGHT+con-si)