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. Author manuscript; available in PMC: 2021 Mar 4.
Published in final edited form as: Neuron. 2019 Dec 23;105(5):909–920.e5. doi: 10.1016/j.neuron.2019.11.024

Figure 1. Genetic isolation of VTA sub-populations.

Figure 1.

(A) Reference atlas images illustrating VTA subregions: rostral VTA (VTAR) – black; parabrachial pigmented nucleus (PBP) – green; paranigral nucleus (PN) – blue. (B) Example images of VTA sections co-stained for YFP (identifying VTA subpopulations after injection with AAV1-FLEX-YFP) and tyrosine hydroxylase (TH). Scale = 100μm. (C) Cell count quantification by VTA subregion (n=3 mice, 9 sections/animal; one-way ANOVA Crhr1: F(2,8)=7.325, P<0.05; Cck: F(2,8)=7.56, P<0.05, Tukey’s multiple comparisons *P < .05). (D) Proportional overlap between YFP and TH. Data represented as mean +/− S.E.M.