Table 2.
Summary of infection results for the tomato leaf curl New Delhi virus (ToLCNDV) isolates, pseudorecombinants, and mutants
| DNA‐Aa | DNA‐B | Agroinoculation | Mechanical inoculationc | ||||
|---|---|---|---|---|---|---|---|
| Nicotiana benthamiana | Oriental melon | Cucumber | Nicotiana benthamiana | Oriental melon | Cucumber | ||
| pCB2A | pCB2B | 10/10 | 11/12 (91.7%) | 11/12 (91.7%) | 0/26 | 0/25 | 0/24 |
| pOM2A | pOM2B | 10/10 | 11/12 (91.7%) | 10/12 (83.3%) | 22/26 (84.6%) | 10/25 (40.0%) | 11/25 (44.0%) |
| pCB2A | pOM2B | 10/10 | 11/12 (91.7%) | 12/12 | 19/26 (73.1%) | 11/26 (42.3%) | 10/27 (37.0%) |
| pOM2A | pCB2B | 9/10 | 10/12 (83.3%) | 11/12 (91.7%) | 0/26 | 0/25 | 0/25 |
| pOM2A | pOM1B::NSP ins39 | 5/5 | 2/5 (40.0%) | ‐ | 6/6 | 5/8 (62.5%) | ‐ |
| pCB2A | pCB1B | 10/10 | 15/17 (88.2%) | 10/11 (90.9%) | 0/26 | 0/30 | 0/28 |
| pOM2A | pOM1B | 10/10 | 11/13 (84.6%) | 13/13 | 21/26 (80.8%) | 17/29 (58.6%) | 14/27 (51.9%) |
| pCB2A | pCB1B::OMNSP | 9/10 (90.0%)b | 11/13 (84.6%) | 10/12 (83.3%) | 0/26 | 0/29 | 0/27 |
| pOM2A | pOM1B::CBNSP | 10/10 | 12/13 (92.3%) | 12/13 (92.3%) | 22/26 (84.6%) | 16/29 (55.2%) | 12/27 (44.4%) |
| pCB2A | pCB1B::OMMP | 12/12 | 17/17 | 10/11 (90.9%) | 23/26 (88.5%) | 14/30 (46.7%) | 12/30 (40.0%) |
| pOM2A | pOM1B::CBMP | 10/10 | 12/13 (92.3%) | 13/13 | 0/26 | 0/29 | 0/27 |
| pCB2A | pCB1B::OM5′ MP | 8/8 | –d | – | 7/8 (87.5%) | 4/8 (50.0%) | 3/8 (37.5%) |
| pCB2A | pCB1B::OM3′ MP | 8/8 | – | – | 0/8 | 0/8 | 0/8 |
| pOM2A | pOM1B::CB5′ MP | 8/8 | – | – | 0/8 | 0/8 | 0/8 |
| pOM2A | pOM1B::CB3′ MP | 8/8 | – | – | 6/8 (75.0%) | 3/8 (37.5%) | 4/8 (50.0%) |
| pCB2A | pCB1BMP (3I→T) | 8/8 | – | – | 0/8 | 0/9 | 0/5 |
| pCB2A | pCB1BMP (6D→E) | 8/8 | – | – | 0/8 | 0/12 | 0/12 |
| pCB2A | pCB1BMP (8V→M) | 8/8 | – | – | 0/8 | 0/9 | 0/5 |
| pCB2A | pCB1BMP (19G→E) | 8/8 | – | – | 7/8 (87.5%) | 6/9 (66.7%) | 1/5 (20.0%) |
| pOM2A | pOM1BMP (3T→I) | 8/8 | – | – | 8/8 | 7/9 (77.8%) | 1/5 (20.0%) |
| pOM2A | pOM1BMP (6E→D) | 8/8 | – | – | 7/8 (87.5%) | 3/12 (25.0%) | 9/12 (75.0%) |
| pOM2A | pOM1BMP (8M→V) | 8/8 | – | – | 7/8 (87.5%) | 6/9 (66.7%) | 1/5 (20.0%) |
| pOM2A | pOM1BMP (19E→G) | 8/8 | – | – | 0/8 | 0/9 | 0/5 |
Abbreviations for amino acids: G, glycine; E, glutamate; I, isoleucine; T, threonine; D, aspartic acid; V, valine; M, methionine.
a
Detailed construction maps of the infectious DNA‐A and ‐B are illustrated in Figures 1a, 3a, 4a and 5.
b
Disease incidence (%) is indicated by number of infected plants/total number of plants inoculated.
c
The sap was prepared from the agroinoculated N. benthamiana and used as inoculum for mechanical inoculation.
d
The symbol “–“ indicates that infectivity was not determined.