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Primers used for construction of mutant and complemented strains
| Primers | Sequence (5′–3′, restriction enzyme sites are underlined) | Restriction enzyme | Product of PCR (bp) |
|---|---|---|---|
| LacrR‐F | GAATTCCGAGCCAGCGCATCAT | EcoRI | 472 |
| LacrR‐R | GGATCCGGCAATTCTCCTGGGTC | BamHI | |
| RacrR‐F | GTCGACCGCCCCGCCGTCCTAT | SalI | 541 |
| RacrR‐R | AAGCTTCATGTCGGTCGGCTTCG | HindIII | |
| acrR‐F | CCCAAGCTTATGATCCACGTCGTGC | HindIII | 630 |
| acrR‐R | CGGGATCCTCACACCAGCTGGTTG | BamHI | |
| GmF | GGATCCCGACGCACACCGTGGAAA | BamHI | 855 |
| GmR | GTCGACGCGGCG TTGTGACAATTT | SalI |
