Table 2.
Bacterial strains and plasmids used in this study
| Strain or plasmid | Description | Reference or source |
|---|---|---|
| Strains | ||
| Escherichia coli | ||
| DH5α | supE44 ΔlacU169(Φ80lacZ ΔM15) hsdR17 recA1 endA1 gyrA96 thi‐1 relA1 | Hanahan, (1983) |
| Acidovorax citrulli | ||
| Aac‐5 | Wild‐type watermelon strain; Apr | Yan et al. (2013) |
| ΔacrR | acrR mutant, Apr; Gmr | This study |
| ΔacrRcomp | ΔacrR complementation strain, ΔacrR containing pBBR‐acrR; Apr, Gmr, Kmr | This study |
| Plasmids | ||
| pK18mobsacB | Cloning and suicide vector with sacB for mutagenesis; Kmr | Schäfer et al. (1994) |
| pK18‐acrR‐Up&Down | pK18mobsacB carrying 472‐ and 541‐bp upstream and downstream sequences of acrR; Kmr | |
| pK18‐acrRGm | pK18mobsacB carrying 630‐bp coding region of acrR replaced by 855‐bp Gm cassette, as well as 472‐ and 541‐bp upstream and downstream sequences of the gene; Kmr | This study |
| pBBR1MCS‐2 | Broad‐host range expression vector; Kmr | Kovach et al. (1994) |
| pBBR‐acrR | pBBR1MCS‐2 carrying 630‐bp coding region of acrR; Kmr | This study |
Kmr, Apr, and Gmr indicate resistance to kanamycin, ampicillin, and gentamicin, respectively.