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. 2020 Feb 22;21(4):445–459. doi: 10.1111/mpp.12894

Figure 4.

Figure 4

SCRE1 suppresses different types of immunity‐associated hypersensitive cell death in Nicotiana benthamiana. (A) Co‐expression of SCRE1 or truncated SCRE1 without signal peptide (SCRE1‐SP) suppressed BAX‐ (left panel) and INF1‐ (right panel) triggered hypersensitive cell death in N. benthamiana. (B) SCRE1/SCRE1‐SP expression, in contrast to green fluorescent protein (GFP) expression, significantly suppressed BAX‐ and INF1‐induced electrolyte leakage in N. benthamiana. Different letters over error bars indicate significant difference in BAX‐ and INF1‐induced electrolyte leakage of N. benthamiana leaves between SCRE1/SCRE1‐SP co‐expression and GFP co‐expression (P < 0.05). (C) SCRE1‐FLAG and GFP‐FLAG co‐expression imposed no evident effect on BAX‐FLAG expression in N. benthamiana leaves. Upper panel: the proteins isolated from the agroinfiltrated leaves were detected via immunoblotting with anti‐FLAG antibody (α‐FLAG). The bands of SCRE1‐FLAG, GFP‐FLAG and BAX‐FLAG are indicated by arrows. Lower panel: protein loading is indicated by Ponceau S staining.