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. 2020 Mar 6;10:4197. doi: 10.1038/s41598-020-61098-9

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Characterization of sEV isolated from human, rat and mouse serum by Exoquick precipitation. (A) Transmission electron microscopy image of the isolated particles, bar = 200 nm, representative image of sEVs from rat. (B) Representative NTA size distribution profiles of isolated particles and their concentrations. (C) The expression of CD63, CD81 and CD9, as well as calnexin and albumin in the isolated particles was determined by western blotting. Lanes from left to right are serum sEV, de-sEV serum and whole serum, respectively. The images are the representatives of three or more independent experiments. Full-length blots/gels are presented in Supplementary Fig S2. sEV, small extracellular vesicle; de-sEV, small extracellular vesicle depleted.