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. 2020 Mar 6;10:4197. doi: 10.1038/s41598-020-61098-9

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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The length distribution and concentration of small RNAs in serum sEV and de-sEV serum samples from human, rats and mice as detected by Agilent 2100 Bioanalyzer using small RNA chips. (A) Gel-like image of all the samples with biological replicates. (B) For each group, electropherograms for one of the two biological replicates was provided. (C) The concentrations of small RNA (0–296nt) in serum sEV from human, rat and mouse, human vs. rat, ***P = 0.0003; human vs. mouse, **P = 0.0053; rat vs. mouse, *P = 0.0159; de-sEV serum, human vs. rat, P > 0.05, ns; human vs. mouse, **P = 0.0052; rat vs. mouse, *P = 0.0397; the concentrations of small RNA in serum sEV vs. de-sEV serum, human, P > 0.05, ns; rat, P > 0.05, ns; mouse, *P = 0.0134. sEV, small extracellular vesicle; de-sEV, small extracellular vesicle depleted.