Figure 2.
p53 mRNA expression in the p53 mutant mice created by engineered nucleases. (a) Targeting strategy using a pair of TALENs on the p53 gene. Targeting loci of two different TALENs are indicated with red arrows on exon 2 of the p53 gene with their sequences also shown in red, determined by Sanger sequencing of PCR products from WT and p53 mutant mice. The dash (-) denotes a deleted nucleotide; Δ1 denotes the number of deleted nucleotide(s), aa represents amino acid. (b, c) Expression of protein or mRNA encoding p53 in mutant (Mu) MEFs induced upon exposure to 10 J/m2 UV treatment, determined by Western blot analysis and qPCR, respectively. Actin was used as an internal control. The qPCR experiments were performed in triplicates, and error bars indicate means ± standard deviation (SD). Full-length blots are depicted in Supplementary Fig. 6.