Fig. 7. Suppression of DJ-1 enhances ferroptosis-based antitumor therapy in vivo.

a The mice were injected subcutaneously with indicated H1299 cells (#1 sequence of DJ-1 KD was used here) and treated with piperazine erastin (PE; 30 mg/kg, once every other day) through tail vein injection for 16 days. The tumor volume was recorded daily, and the relative tumor volume (RTV) was calculated (n = 6). RTV was shown as the mean ± SEM. b Representative images of tumors in each treatment group. c Representative immunohistochemical image of Ki67 in tumor sections are shown. The experiment was repeated twice independently with similar results. d The TUNEL-positive ratios of tumor regions were analyzed. And the representative images of TUNEL staining in the tumor tissues in different groups are shown. The experiment was repeated twice independently with similar results. e The mRNA expression of PARK7 and PTGS2 in indicated H1299 cells was assayed by qRT-PCR. The relative gene expression was normalized to β-actin. f The 4-HNE levels in indicated tumors were assayed by ELISA. g The Hcy levels in indicated tumors were assayed by ELISA. n = 6 biologically independent samples per group. Comparisons were made using the two-tailed, unpaired Student’s t-test; *p < 0.05, **p < 0.01, ***p < 0.001.