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. 2020 Mar 6;10:4258. doi: 10.1038/s41598-020-61157-1

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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RNAi silencing efficiency of TmRelish mRNA in T. molitor larvae and survival in the 10 days following microbial challenge. qRT-PCR-based estimation of TmRelish knockdown relative to EGFP control, 3 days post-dsRNA injection (A). Effects of TmRelish mRNA knockdown on the survival of Tenebrio molitor larvae post Escherichia coli (B), Staphylococcus aureus (C), and Candida albicans (D) infection. dsEGFP-treated groups infected with the same microbes were used as negative controls. Survival was monitored for 10 d. The experiment was performed thrice with similar results. ‘*’ indicates significant differences between dsTmRelish and dsEGFP-treated groups (p < 0.05).