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. 2020 Mar 6;10:4258. doi: 10.1038/s41598-020-61157-1

Table 1.

Sequences of the primers used in the study.

Primer Sequence (5′-3′)

TmRelish_qPCR_Fw

TmRelish_qPCR_Rv

5′-AGCGTCAAGTTGGAGCAGAT-3′

5′-GTCCGGACCTCATCAAGTGT-3′

TmRelish_Temp_Fw

TmRelish_Temp_Rv

5′-TGTGGGAAGATTACGGGAAA-3′

5′-CAAATTGGCCACGATCTCTT-3′

dsTmRelish_Fw

dsTmRelish_Rv

5′-TAATACGACTCACTATAGGGT

GACGTGCACCATCAATA-3′

5′-TAATACGACTCACTATAGGGT

GCGTGTTTGGCCTTGAT-3′

dsEGFP_Fw

dsEGFP_Rv

5′-TAATACGACTCACTATAGGG

TACGTAAACGGCCACAAGTTC-3′

5′-TAATACGACTCACTATAGGG

TTGCTCAGGTAGTGTTGTCG-3′

TmTenecin-1_Fw

TmTenecin-1_Rv

5′-CAGCTGAAGAAATCGAACAAGG-3′

5′-CAGACCCTCTTTCCGTTACAGT-3′

TmTenecin-2_Fw

TmTenecin-2_Rv

5′-CAGCAAAACGGAGGATGGTC-3′

5′-CGTTGAAATCGTGATCTTGTCC-3′

TmTenecin-3_Fw

TmTenecin-3_Rv

5′-GATTTGCTTGATTCTGGTGGTC-3′

5′-CTGATGGCCTCCTAAATGTCC-3′

TmTenecin-4_Fw

TmTenecin-4_Rv

5′-GGACATTGAAGATCCAGGAAAG-3′

5′-CGGTGTTCCTTATGTAGAGCTG-3′

TmDefensin-1_Fw

TmDefencin-1_Rv

5′-AAATCGAACAAGGCCAACAC-3′

5′-GCAAATGCAGACCCTCTTTC-3′

TmDefensin-2_Fw

TmDefensin-2_Rv

5′-GGGATGCCTCATGAAGATGTAG-3′

5′-CCAATGCAAACACATTCGTC-3′

TmColeoptericin-1_Fw

TmColeoptericin-1_Rv

5′-GGACAGAATGGTGGATGGTC-3′

5′-CTCCAACATTCCAGGTAGGC-3′

TmColeoptericin-2_Fw

TmColeoptericin-2_Rv

5′-GGACGGTTCTGATCTTCTTGAT-3′

5′-CAGCTGTTTGTTTGTTCTCGTC-3′

TmAttacin-1a_Fw

TmAttacin-1a_Rv

5′-GAAACGAAATGGAAGGTGGA-3′

5′-TGCTTCGGCAGACAATACAG-3′

TmAttacin-1b_Fw

TmAttacin-1b_Rv

5′-GAGCTGTGAATGCAGGACAA-3′

5′-CCCTCTGATGAAACCTCCAA-3′

TmAttacin-2_Fw

TmAttacin-2_Rv

5′-AACTGGGATATTCGCACGTC-3′

5′-CCCTCCGAAATGTCTGTTGT-3′

TmCecropin-2_Fw

TmCecropin-2_Rv

5′-TACTAGCAGCGCCAAAACCT-3′

5′-CTGGAACATTAGGCGGAGAA-3′

TmThaumatin-like protein-1_Fw

TmThaumatin-like protein-1_Rv

5′-CTCAAAGGACACGCAGGACT-3′

5′-ACTTTGAGCTTCTCGGGACA-3′

TmThaumatin-like protein-2_Fw

TmThaumatin-like protein-2_Rv

5′-CCGTCTGGCTAGGAGTTCTG-3′

5′-ACTCCTCCAGCTCCGTTACA-3′

TmL27a_qPCR_Fw

TmL27a_qPCR_Rv

5′-TCATCCTGAAGGCAAAGCTCCAGT-3′

5′-AGGTTGGTTAGGCAGGCACCTTTA-3′

Underlined region indicates T7 promoter sequences.