Table 1.
Primer | Sequence (5′-3′) |
---|---|
TmRelish_qPCR_Fw TmRelish_qPCR_Rv |
5′-AGCGTCAAGTTGGAGCAGAT-3′ 5′-GTCCGGACCTCATCAAGTGT-3′ |
TmRelish_Temp_Fw TmRelish_Temp_Rv |
5′-TGTGGGAAGATTACGGGAAA-3′ 5′-CAAATTGGCCACGATCTCTT-3′ |
dsTmRelish_Fw dsTmRelish_Rv |
5′-TAATACGACTCACTATAGGGT GACGTGCACCATCAATA-3′ 5′-TAATACGACTCACTATAGGGT GCGTGTTTGGCCTTGAT-3′ |
dsEGFP_Fw dsEGFP_Rv |
5′-TAATACGACTCACTATAGGG TACGTAAACGGCCACAAGTTC-3′ 5′-TAATACGACTCACTATAGGG TTGCTCAGGTAGTGTTGTCG-3′ |
TmTenecin-1_Fw TmTenecin-1_Rv |
5′-CAGCTGAAGAAATCGAACAAGG-3′ 5′-CAGACCCTCTTTCCGTTACAGT-3′ |
TmTenecin-2_Fw TmTenecin-2_Rv |
5′-CAGCAAAACGGAGGATGGTC-3′ 5′-CGTTGAAATCGTGATCTTGTCC-3′ |
TmTenecin-3_Fw TmTenecin-3_Rv |
5′-GATTTGCTTGATTCTGGTGGTC-3′ 5′-CTGATGGCCTCCTAAATGTCC-3′ |
TmTenecin-4_Fw TmTenecin-4_Rv |
5′-GGACATTGAAGATCCAGGAAAG-3′ 5′-CGGTGTTCCTTATGTAGAGCTG-3′ |
TmDefensin-1_Fw TmDefencin-1_Rv |
5′-AAATCGAACAAGGCCAACAC-3′ 5′-GCAAATGCAGACCCTCTTTC-3′ |
TmDefensin-2_Fw TmDefensin-2_Rv |
5′-GGGATGCCTCATGAAGATGTAG-3′ 5′-CCAATGCAAACACATTCGTC-3′ |
TmColeoptericin-1_Fw TmColeoptericin-1_Rv |
5′-GGACAGAATGGTGGATGGTC-3′ 5′-CTCCAACATTCCAGGTAGGC-3′ |
TmColeoptericin-2_Fw TmColeoptericin-2_Rv |
5′-GGACGGTTCTGATCTTCTTGAT-3′ 5′-CAGCTGTTTGTTTGTTCTCGTC-3′ |
TmAttacin-1a_Fw TmAttacin-1a_Rv |
5′-GAAACGAAATGGAAGGTGGA-3′ 5′-TGCTTCGGCAGACAATACAG-3′ |
TmAttacin-1b_Fw TmAttacin-1b_Rv |
5′-GAGCTGTGAATGCAGGACAA-3′ 5′-CCCTCTGATGAAACCTCCAA-3′ |
TmAttacin-2_Fw TmAttacin-2_Rv |
5′-AACTGGGATATTCGCACGTC-3′ 5′-CCCTCCGAAATGTCTGTTGT-3′ |
TmCecropin-2_Fw TmCecropin-2_Rv |
5′-TACTAGCAGCGCCAAAACCT-3′ 5′-CTGGAACATTAGGCGGAGAA-3′ |
TmThaumatin-like protein-1_Fw TmThaumatin-like protein-1_Rv |
5′-CTCAAAGGACACGCAGGACT-3′ 5′-ACTTTGAGCTTCTCGGGACA-3′ |
TmThaumatin-like protein-2_Fw TmThaumatin-like protein-2_Rv |
5′-CCGTCTGGCTAGGAGTTCTG-3′ 5′-ACTCCTCCAGCTCCGTTACA-3′ |
TmL27a_qPCR_Fw TmL27a_qPCR_Rv |
5′-TCATCCTGAAGGCAAAGCTCCAGT-3′ 5′-AGGTTGGTTAGGCAGGCACCTTTA-3′ |
※Underlined region indicates T7 promoter sequences.