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. 2020 Mar 6;11(3):175. doi: 10.1038/s41419-020-2268-8

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 6 — a, b Transfection efficiency analysis of siATG12 in SW480 and HCT116 cells through western blot assay at 48 h after transfection. c–h The effects of ATG12 knockdown on cell radiosensitivity, viability, and apoptosis were measured by radiation clonogenic survival assay (c, d), CCK-8 assay (e, f), and flow cytometry analysis (g, h). i The effects of ATG12 silencing alone or in combination with IR stimulation on protein expression of LC3 I, LC3 II, p62, cleaved caspase 3, Bax, and Bcl-2 were measured by western blot assay. *P < 0.05.