Fig. 7. HOTAIR knockdown enhanced radiosensitivity of CRC xenograft tumors.

a–d CRC xenograft tumor models were established by subcutaneous injection of SW480 cells infected with control lentiviruses or shHOTAIR lentiviruses into the flanks of mice. Ten days later, mice were irradiated with 10 Gy of IR once. a Tumor volume was monitored using a caliper at day 7, 10, 13, 16, 19, 22, 25, and 28 after injection. b Tumor weight was measured at day 28 after injection. c HOTAIR and miR-93 levels in CRC xenograft tumors were determined by RT-qPCR assay. d Protein expression patterns of ATG12, LC3 II, and cleaved caspase 3 were evaluated by IHC assay. *P < 0.05.