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. 2020 Mar 6;10:4195. doi: 10.1038/s41598-020-60815-8

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Investigator Assessments of the study patients. (A–D) Visual Analogue Scale for Tactile Assessment. The lines indicate the 10-cm VAS scale ranging from −50 to +50. Negative values indicate a harder and smoother surface, and positive values indicate softer and rougher surface. Top Control Group (blue), middle Test Group 1 (green), bottom Test Group 2 (orange). The mean value is indicated by a vertical line, and the bars indicate the 95% Confidence Interval. (A) Baseline; (B) Day 90; (C) Day 180; (D) Day 360. (E) Bar chart representing the number of answers to the Global Impression of Change questionnaire for Control, Test Group 1 and Test Group 2 on the indicated follow-ups for each answer. The answers are colour coded: Worse (red); A little worse (light red); Unchanged (grey); A little better (light green); Better (green); Very much better (dark green); Missing answers (black).