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. 2020 Mar 6;10:4204. doi: 10.1038/s41598-020-60993-5

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Un-rooted phylogenetic tree of 24-sterol reductases in diatoms, plants, algae, fungi and animals. Reactions catalyzed by each sub-family are shown next to the enzymatic groups. Alignment used maximum likelihood method. The bootstrapping test (replicate = 100) is reported on each node. Red square = S. marinoi; Green square = C. cryptica; Light blue spot = other diatom. DAWRF1 = plant 24-sterol reductase; SSR = plant sterol side chain reductase; 24-DHCR = animal 24-dehydrocholesterol reductase; 24(28)SR/ERG4 = fungal Delta(24(24)1))-sterol reductase.