Table 2.
Transcript sequences of genes coding for enzymes of sterol biosynthetic pathway in S. marinoi and C. cryptica.
| Biosynthetic Function | Predicted enzymatic function by Blast2GO | S. marinoi transcripts | Expression (FPKM counts) | C. cryptica transcripts | Expression (FPKM counts) | |||||
|---|---|---|---|---|---|---|---|---|---|---|
| Replicate 1 | Replicate 2 | Replicate 3 | Replicate 1 | Replicate 2 | Replicate 3 | |||||
| Terpene elongation and cyclization | Farnesyl pyrophosphate synthetase | FPPS | TR5774 | 747 | 1469 | 2779 | TR24671 | 1580 | 1363 | 1796 |
| Squalene synthase | SQS | TR5837 | 7653 | 9077 | 10903 | TR905 | 7731 | 11473 | 7648 | |
| Alternative Squalene Epoxidase | AltSQE | TR7561 | 7708 | 1005 | 1765 | TR29442 | 13916 | 16726 | 10147 | |
| Cycloartenol synthase | CAS | TR12960 | 4902 | 5597 | 5266 | TR3126 | 2825 | 3011 | 3105 | |
| Construction of the sterol skeleton | Cycloartenol-C-24-methyltransferase -1 | SMT1 | TR612 | 399669 | 547431 | 495121 | TR44522 | 4872 | 4562 | 10006 |
| Cycloartenol-C-24-methyltransferase -2 | SMT1 | — | — | — | — | TR45147 | 34639 | 25720 | 33901 | |
| Sterol Methyl transferase | SMT2 | TR11812 | 19023 | 13794 | 15818 | TR29102 | 66731 | 62025 | 70507 | |
| Sterol Methyl transferase | SMT2 | TR21333 | 24344 | 13997 | 14326 | TR17021 | 33805 | 42406 | 37980 | |
| C-4 Methylsterol oxidase | 3βHSD-D | TR28093 | 2287 | 2523 | 1960 | — | — | — | — | |
| Sterol-4-methyl oxidase 1 | SMO1 | TR11964 | 40534 | 37090 | 35927 | TR32604 | 20979 | 29827 | 25702 | |
| Sterol-4-methyl oxidase 1 | SMO1 | TR30213 | 3061 | 3258 | 2926 | TR28329 | 0.000 | 1731 | 2017 | |
| Sterol-4-methyl oxidase 2 | SMO2 | TR319 | 17019 | 18736 | 18676 | TR32604 | 20979 | 29827 | 25702 | |
| Sterol-4-alpha-carboxylate 3-dehydrogenase | ERG26/D4C | TR28078 | 48329 | 29541 | 39087 | TR14638 | 11001 | 7800 | 11499 | |
| Cycloeucalenol cycloisomerase | CPI | TR11937 | 8673 | 8864 | 8943 | TR30071 | 3081 | 3643 | 4002 | |
| Sterol 14 demethylase -1 | CYP | TR7602 | 5822 | 3161 | 3628 | TR12199 | 32581 | 38398 | 40078 | |
| Sterol 14 demethylase -2 | CYP | — | — | — | — | TR23734 | 6396 | 4710 | 5582 | |
| Sterol 14 demethylase -3 | CYP | — | — | — | — | TR30096 | 70903 | 65490 | 74001 | |
| Delta 14 sterol reductase | C14SR | TR13312 | 5630 | 5771 | 5383 | TR19404 | 47799 | 46207 | 50117 | |
| Delta 5 Sterol desaturase | Δ5 SD/DWARF7 | TR28093 | 2287 | 2523 | 1960 | TR48724 | 0.000 | 7168 | 2531 | |
| 7 Dehydrocholesterol reductase | Δ7SR/DWARF5 | TR2636 | 7653 | 17187 | 13127 | TR24068 | 10801 | 9765 | 9443 | |
| Delta(24(24(1)))-sterol reductase | 24SR/ERG4 | TR213 | 14750 | 18308 | 18373 | TR29411 | 88345 | 78049 | 66050 | |
| 24 dehydrocholesterol reductase -2 | 24-DHCR | TR10998 | 45299 | 64582 | 64882 | — | — | — | — | |
| 22 sterol desaturase | ERG5 | — | — | — | — | TR3228 | 11535 | 12460 | 13684 | |