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. 2020 Mar 6;10:4247. doi: 10.1038/s41598-020-61192-y

Figure 7.

Figure 7

The intestinal microbiota induces the expression of interleukins, Toll-like receptor 5 and the lectin RegIIIα. The capacity of microbial fecal cells (MFCs) extracted from 22 omnivores (O), 20 vegetarians (VG) and 19 vegans (V) and the corresponding microbial protein cell extracts (MPCEs) and flagellin (FlC) at two different concentrations (0.015 µg/ml of DMEM for O and 0.090 µg/ml for VG and V MPCE samples) to affect the expression of IL-8 (panels A–C), IL-22 (D–F) and IL-23 (G–I), Toll-like receptor 5 (J–L) and the lectin RegIIIα (M–O) was assessed at 6 and 24 h in human HT29 colon cancer cells. The levels of expression of each gene in colon cancer cells treated with MFCs, MPCEs or FlC, compared to control (cells treated with PBS only), presented as the mean value ± s.d. from three replicates for each of the 61 samples. All data shown are representative of three independent experiments using fecal samples collected at three time-points in one month. Differences between control and treated cells were considered statistically significant when p < 0.05. A schematic representation of how interleukins, TLR-5 and RegIIIα work synergically is provided in panel P.