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. 2020 Mar 6;10:4218. doi: 10.1038/s41598-020-61079-y

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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The effects of storage conditions on porcine tissue properties. (A) Corneal endothelium density of fresh ocular tissue (n = 19 biological replicates, left axis) as determined by specular microscopy and corneal apex thickness of fresh ocular tissue (n = 20 biological replicates, right axis) as determined by optical coherence tomography (OCT). (B) Comparison of corneal thickness for fresh tissue vs. tissue stored at 4 °C, −20 °C, −80 °C for 1 or 7 days. Statistical significance determined by one-way ANOVA, post-hoc Dunnett’s multiple comparison test. (C) Comparison of ocular compliance for fresh vs. preserved tissue after 20 µL volume injection. Statistical significance determined by one-way ANOVA, post-hoc Dunnett’s multiple comparison test. (D) Outflow rate at 15 mmHg for fresh vs. preserved tissue (n = 3 biological replicates for each). Statistical significance determined by Kruskal-Wallis test, post-hoc Dunn’s multiple comparisons test. Error bars denote standard deviation.