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. 2020 Mar 6;10:4270. doi: 10.1038/s41598-020-61024-z

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Breastmilk HMO profiling. (a) PCA showing separate clustering of breastmilk samples of 24 mothers at two, six and 12 weeks of lactation, also indicating mother’s secretor status. The 3FL, LNFP III, and 3SL (3SL between week six and 12 only) are positively associated with duration of lactation; (b) Average concentration of different HMOs found in the breastmilk samples from the 24 mothers included in the study at two, six and 12 weeks of lactation; (c) Estimated average daily intake of each HMO in the 24 infants at two, six and 12 weeks of age.