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. 2020 Mar 6;11(3):173. doi: 10.1038/s41419-020-2361-z

Fig. 3. miR-450a-5p inhibited SOX2 expression by directly targeting its 3′UTR.

Fig. 3

The data from RNA sequencing and public database (microRNA, TargetScan and miRWalk) were overlapped using Venny tool (a). qRT-PCR was utilized to further detected the expression of possible miRNAs in CRC cells (b). miR-450a-5p and its putative binding sequences in the 3′UTR of SOX2. A mutation was generated in the complementary site that binds to the seed region of miR-450a-5p. A wild/mutant type SOX2 3′UTR reporter (or control construct) and miR-450a-5p plasmid (or control plasmid) were transduced into 293T cells, and luciferase activity was assessed (c). qRT-PCR and western blot were used to determine miR-450a-5p and SOX2 expression in 8 paired human colorectal cancer, respectively (d). All data are represented as fold-change±SD compared to control cells or group. Experiments were performed in triplicate. *P < 0.05.