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. 2020 Mar 6;10:4239. doi: 10.1038/s41598-020-61054-7

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Experimental setup. Schematic overview of the experimental procedure and associated timeline for culturing Caenorhabditis elegans and its bacterial food source (Escherichia coli OP 50). The timeline for preparing and incubating the bioassay plate, as well as for C. elegans oxygen consumption rate (OCR) measurement using the Seahorse XF^e96 Extracellular Flux Analyzer, is also provided.