Fig. 6. Models depicting how LC8 binding facilitates polymerase activity.

In the absence of LC8 (right), the CTDs (green ovals) occupy both distal and proximal conformations, leading to non-ideal movement of the polymerase along the RavN^RNA complex (maroon). When RavP is bound to LC8 (left), CTDs are forced into a proximal conformation, facilitating polymerase movement along the RavN^RNA complex and leading to increased polymerase efficiency, evinced by the minigenome assay comparison of RavP-WT to RavP-AAA (Fig. 5D).