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rPMCs exhibit phenotype engaged in response to viral infection. Constitutive expression of TLR3, IRF3, IFNAR1, IFNGR1, and MHC I proteins in cell lysates analyzed by Western blot. β-Actin was used as a loading control (a). Constitutive expression of TLR3 (surface and intracellular), IRF3, IFNAR1, IFNGR1, and MHC I molecules analyzed by flow cytometry. Shaded areas indicate staining with isotype-matched control antibodies (b). Data are representative of 3 independent experiments.
