Figure 5.

TLR3 ligand-induced release of de novo synthesized mediators from rPMCs is TLR3- and NF-κB-dependent. rPMCs were preincubated with medium alone (control), anti-TLR3 antibodies (40 μg/mL), or isotype control antibodies for 1 h and after rinsing exposed to poly(I:C) at 10 μg/mL for 2 h (cysLT release) or 12 h (cytokine release) (a). rPMCs were preincubated with medium alone (control) or MG-132 (3 μM) for 15 min and after rinsing treated with poly(I:C) at 10 μg/mL for 2 h (cysLT release) or 12 h (cytokine release) (b). Results are reduced by the value of the spontaneous release. Data are expressed as the mean ± SD of 4 independent experiments, and each experiment was done in duplicate (n = 8). ^∗p < 0.05, ^∗∗p < 0.01, and ^∗∗∗p < 0.001; n.s.: not significant.