Figure 6.

Effect of ZR30 on glioma cell growth. A, CCK‐8 colorimetric assay for the determination of cell growth, measured daily (for 97B) or every 3 days (for 97A and 98A) for absorbance at 450 nm by cells (1000 cells/well in 96‐well plate with 5 replicates in 0.1 mL culture medium with or without ZR30), which were changed after CCK‐8 assay. B, Cell doubling time calculated based on the number of viable cell with exclusion of Trypan blue (0.02%), counted 1 week after seeding 20 000 cells/well in a 12‐well plate with 3 replicates in 1 mL culture medium with or without ZR30. The assay was repeated on cells with or without 2 weekly passages