Table 1.
Gene abnormality and short tandem repeat (STR) profiles of syngeneic glioblastoma multiforme (GBM) primary cultures
| Tumor | Histology | Clinical data | Established cultures | Culture condition | Mutation, deletion, or amplification of genes | ||||
|---|---|---|---|---|---|---|---|---|---|
| TP53 | IDH1 | IDH2 | PTEN | EGFR | |||||
| GBM51 | GBM | 61 y, F, recurrent | 51A | NS | wt | wt | wt | wt | High amp |
| 51B | SA | wt | wt | wt | wt | No amp | |||
| STR: AMEL(X), CSF1PO(10, 12), D13S317(11, 12), D16S539(11, 13), D18S51(12, 15), D21S11(29), D3S1358(13, 15), D5S818(10, 11), D7S820(9), D8S1179(14), FGA(20), Penta D(11, 12), Penta E(5, 10), TH01(8, 9), TPOX(8), vWA(14) | |||||||||
| GBM98 | GBM | 70 y, F, de novo | 98A | NS | L257V | wt | wt | Homozygous p.Q298 trunc | No amp |
| 98B | SA | L257V | wt | wt | Homozygous p.Q298 trunc | No amp | |||
| 98E | EC | L257V | wt | wt | Homozygous p.Q298 trunc, heterozygous pR55 trunc | Low amp | |||
| STR: AMEL(X), CSF1PO(9, 10), D13S317(11), D16S539(12, 13), D18S51(17, 19), D21S11(30, 31.2), D3S1358(16, 17), D5S818(11, 12), D7S820(8, 11), D8S1179(12, 13), FGA(20), Penta D(8, 14), Penta E(13, 15), TH01(6, 9), TPOX(8, 10), vWA(16) | |||||||||
| GBM97 | GBM‐O | 45 y, M secondary | 97A | NS | wt | wt | wt | Homozygous deletion | Low amp |
| 97B | SA | wt | wt | wt | Homozygous deletion | Low amp | |||
| STR: AMEL(X, Y), CSF1PO(12, 13), D13S317(11), D16S539(12, 13), D18S51(14, 16), D21S11(31, 32.2), D3S1358(15, 18), D5S818(11, 12), D7S820(8, 11), D8S1179(13), FGA(24, 26), Penta D(9, 14), Penta E(14, 15), TH01(6, 8), TPOX(8, 9),vWA(15, 17) | |||||||||
amp, amplification; EC, adherent cultures maintained in endothelial cell growth medium in fibronectin (1 μg/cm2)‐coated dishes; F, female; M, male; NS, cells cultured with DMEM/F12 medium supplemented with epidermal growth factor (20 ng/mL), basic fibroblast growth factor (10 ng/mL), and 5% B27 (Invitrogen), initially in low‐binding or 1% agar‐coated dishes (for 3‐4 weeks), then in adherent conditions in fibronectin (1 μg/cm2)‐coated dishes, and back and forth for at least 2 rounds of adherent and spheroid cultures; SA, cells cultured with DMEM/F12 medium supplemented with 10% FBS in collagen‐coated (initial culture) or regular culture dishes (subsequent passages); trunc, truncated.