Figure 2.

YES1 knockdown overcomes neratinib resistance. A, MTS assay to assess the sensitivity to neratinib of (i) BT‐474, (ii) BT‐474‐NRS, (iii) BT‐474‐NRS2, (iv) H2170 and (v) H2170‐NRH after YES1 knockdown. Cells were transfected with siRNA followed by treatment with neratinib for 72 h. The assay was repeated three times. Data are shown as mean ± SE; *P < 0.05, **P < 0.01 vs negative control (siNEC). B, Effect of YES1 knockdown on the protein expression and phosphorylation profiles in the presence/absence of neratinib treatment in (i) BT‐474, BT‐474‐NRS, BT‐474‐NRS2, (ii) H2170 and H2170‐NRH. The cells were treated with neratinib for 2 h after the siRNA transfection