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. 2020 Feb 15;111(3):795–806. doi: 10.1111/cas.14293

Figure 3.

Figure 3

A, Interleukin (IL)‐33 regulates the expression of CCL2 in esophageal squamous cell carcinoma (ESCC) cells. A, Cytometric bead array (CBA) for changes of cytokines in KYSE‐450 cells overexpressing IL‐33 (OE‐IL‐33) and the control group (NC). B,C, RT‐PCR and ELISA to detect the change of CCL2 level. D, CBA for changes of cytokines in IL‐33 knockdown and control groups. E,F, RT‐PCR and ELISA to detect the change of CCL2 level with knockdown of IL‐33 in Eca‐109 cells. G, RT‐PCR and ELISA to detect the expression of CCL2 level with addition of IL‐33 and knockdown of IL‐33 receptor ST2. H, Western blotting revealed the protein level of active form of nuclear factor (NF)‐κB and CCL2 with knockdown of IL‐33 or its receptor. I, Association between CCL2 and IL‐33 gene levels in ESCC patients. Data are presented as mean ± SD