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. 2020 Mar 6;20:108. doi: 10.1186/s12870-020-2317-6

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© The Author(s). 2020

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 6 — Detection of transcriptional activation of the 4 bHLHs in 14 candidate genes associated with the pear single sigmoid pattern. a Constructed effectors and reporters. b Dual-luciferase assay of effectors on reporters. Cell development-associated genes include CESA (Pbr038537.1), GT (Pbr016727.1, Pbr023514.1, and Pbr023516.2), MAP (Pbr011537.1 and Pbr039779.1), UGT (Pbr018679.1 and Pbr021540.1), CSN (Pbr025986.3), EXOC (Pbr029906.1), EXP (Pbr013129.1), XG GalT (Pbr036086.1 and Pbr004891.1), and XG UGT (Pbr005326.1). * and ** indicate significant correlations (p < 0.05 and p < 0.01, respectively)