Fig. 3.

MiR-7 decreases CD44 expression by directly targeting the 3′UTR of RELA. a Putative miR-7 wild-type and mutated binding sites in RELA. b Luciferase reporter activity. c Representation of CD44 promoter shows six RELA-binding sites d. PCR-ChIP assays. In MDA-MB-231 cells, putative RELA-binding sites were identified at regions − 1234 to − 1243, − 1654 to − 1663, and − 2073 to − 2082 in the CD44 promoter. e–j The RELA and CD44 transcriptional and translational expression levels following siRELA transfection of MDA-MB-231, MCF-7, and SK-BR-3 cells