Fig. 4.

MiR-7/miR-92b specifically bind to XIST in cells. a Putative miR-7 wild-type and mutated binding sites in XIST. b–d Luciferase reporter activity in MDA-MB-231 and LD cells. e Putative miR-92b binding and mutated sites in XIST. f Luciferase reporter activity in MDA-MB-231 and LD cells. g Specific isolation of XIST in MDA-MB-231 cells by RIP assay. h Quantitative analysis of XIST pulldown efficiency in cells. i RT-qPCR analysis of specific isolation of miR-7-5p from the precipitation of the XIST complex in MDA-MB-231 cells. j Quantitative analysis of miR-7-5p pulldown efficiency in the precipitation of the XIST complex in cells