Fig. 2.

Workflow of the two proteomic approaches used in this study. Each protein was extracted from individual rats from the following groups: sham, IR, and TALE. A portion of each protein extract was subjected to 2-D gels as a part of the gel-based method. The remaining portion of each protein sample was subjected to a TMT-based quantitative strategy, such as a non-gel-based method. Equal amounts of each protein were labeled with an individual TMT and combined into one sample. The mixtures were analyzed quantitatively and qualitatively using 2DL-MS/MS. Consequently, reliable data were obtained following filtering using the statistic criteria as mentioned in the Materials and Methods