Table 2.

HME determinants in STM 6070 genome and their comparison with those detected in other Cupriavidus species

Locus a	Proposed gene annotation	IMG Locus tag b A3AGDRAFT_	HM transporter c	Putative substrate	Orthologous genes
					LMG 19424T	STM 6018	UYPR2.512	AMP6	CH34T	N-1	H16
A	hmyFCBA	00876–00879	HME3b	monovalent cations	+	+	+	+	+	+	+
B	nieIC cep nieBA	00906–00910	HME5	nd	+	+	+	+	–	+	+
	chrB1A1	00912–00911	CHR	CrO42−	+	+	+	–	+	–	–
C	czcJ2 hns czcLRS ubiG zntA	00978 (zntA)	P-ATPase	Zn2+	+	+	+	+	+	+	+
D	silP	00999	P-ATPase	Ag	–	–	–	+	–	–	+
	copP	01005	P-ATPase	Cu2+	–	–	+	+	+	–	+
E	copSRABCD	02911–02906	–	nd	+	+	+	+	+	+	+
F	czcJ1I1C1B1A1	03877–03882	HME1	Co2+, Zn2+, Cd2+	+	+	+	+	+	+	+
G	hmvCBA	04395–04397	HME3a	divalent cations	+	+	+	+	+	+	+
H	cupA cupR	04504	P-ATPase	Cu2+	+	+	+	+	+	+	+
I	czcC2B2A2	04975–04973	HME1	nd	+	+	+	+	+	+	+
	nreAB	04978–04977	MFS	Ni2+	–	–	+	+	+	–	–
	ΔchrB 2A2CF exp chrL	04985–04980	CHR	CrO42−	+	+	+	+	+	+	+
	hmxB zneAC zneR hmxS	04993–04989	HME3a	nd	+	+	+	+	+	+	+
J	ΔcopF	05405	P-ATPase	Cu2+	+	+	+	+	+	+	+
	silDCBAF	05406–05409	HME4	Cu2+, Ag	+	+	+	+	+	+	+
K	czcDI2C3B3A3	05655–05651	CDF + HME1	Co2+, Zn2+, Cd2+	+	+	+	+	+	+	+
	arsR1IC1C2B1C3H1P	05663–05656	ARC3	nd	+	+	–	+	+	+	+
			+MFS		+	+	–	+	+	+	+
L	arsR2C4B2H2	05853–05856	ARC3	nd	+	+	–	+	+	+	+
Not in HMR clusters	dmeF	04892	CDF + HME1	nd	+	+	–	+	+	+	+
	fieF1	03199	CDF + HME1	nd	+	+	–	+	+	+	+
	fieF2	06164	CDF + HME1	nd	+	+	–	+	+	+	+

Footnote: STM 6070, Cupriavidus sp. STM 6070; LMG 19424T, C. taiwanensis LMG 19424T; STM 6018, C. taiwanensis STM 6018; UYPR2.512, Cupriavidus sp. UYPR2.512; AMP6, Cupriavidus sp. AMP6; CH34T, C. metallidurans CH34T; N-1, C. necator N-1 T

^a, HM loci names from Fig. 2 and Table S6

^b, IMG Locus tag (JGI);

^c, classification of the annotated HM proteins from Table S5