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. 2020 Mar 7;20:75. doi: 10.1186/s12935-020-1154-x

Fig. 3.

Fig. 3

Linc02042 increases c-Myc stability. a qRT-PCR analysis of c-Myc expression in linc02042-silenced ESCC cells. b Western blotting and IHC staining analysis of c-Myc protein expression in linc02042-silenced ESCC cells and transplanted tumor tissues, respectively. c Luciferase reporter assay detecting the promoter activity of c-Myc in linc02042-silenced ESCC cells. d qRT-PCR analysis of remaining c-Myc expression in linc02042-silenced ESCC cells treated with 1 μM Actinomycin D for the indicated time. e qRT-PCR analysis of c-Myc expression in ESCC adjacent normal tissues. f The correlation between linc02042 and c-Myc expression in ESCC tissues. g The assessment of cell viability and invasion respectively detected by CCK-8 and Transwell assays in linc02042-overexpressing cells transfected with c-Myc siRNA. **p < 0.01