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. 2020 Mar 6;20:55. doi: 10.1186/s12866-020-01725-7

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© The Author(s) 2020

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 7 — Overexpression of NbMLP28 in transgenic N. benthamiana has germination and rooting promoting effect. a PCR amplification to selected highly overexpressed transgenic plants, sample 3 was a transgenic tobacco that strongly expresses NbMLP28.b Protein detection of overexpressing plants T4 generation stably expressing RFP-tagged MLP28. c Statistics on germination rate of over-expressed NbMLP28 transgenic tobacco and wild type at 3, 4, 5, and 6 days after seeding. d The fresh weight statistics of over-expression of NbMLP28 transgenic tobacco and wild-type at 12 days after seeding. e Differences in growth status of overexpressed NbMLP28 transgenic tobacco and wild type at 12 days after seeding. f Detection root length of over-expressed NbMLP28 transgenic tobacco and wild-type at 12 days after seeding