Fig. 8.

Identification of NbMLP28 response to the hormonal pathway in N. benthamiana. a Expression of signal transduction key genes NPR1, COI1 and EIN2 of SA, JA and ET after 3 days of virus infection. The data were analyzed with independent sample T test using SPSS Statistics v.21 software, different letters indicate that values of the two treatments were significantly different at P < 0.05 (b) The expression of NbMLP28 after spraying with 0.5 mM SA, 0.1 mM Me JA or 0.05 mM Ethephon, the MOCK was sprayed with water with 0.02% Tween 20. The data were analyzed by Duncan’s multiple range tests in the ANOVA program of SPSS, different letters indicate that values of the four treatments were significantly different at P < 0.05, the same as C and D. c The expression of NbMLP28 after silencing NPR1, COI1 and EIN2. d The expression of PVY-GFP after silencing NPR1, COI1 and EIN2