Fig. 4.

Temporal proteomic analyses of the protein contents found in the insoluble fraction of the PFF-treated neurons reveals a high increase in proteins related to the endomembrane system. (A) Insoluble proteins from neurons treated with PBS and PFFs for 7, 14, and 21 d were extracted and analyzed using LC-MS/MS. Identified proteins were plotted using volcano plot. Dotted lines represent the false discovery rate < 0.05 and threshold of significance SO = 1 assigned for the subsequent analysis. A detailed list of the hits is available in Dataset S1. Classifications of the proteins significantly enriched in the insoluble fractions of the PFF-treated neurons at D14 and D21 by cellular component and biological processes using gene ontology (GO) and DAVID enrichment analyses are shown in SI Appendix, Fig. S8 B and C. (B) Heat maps representing color-coded fold-change levels of mitochondrial (Left) and synaptic (Right) proteins present in the insoluble fraction in PFF-treated neurons.