Fig. 2.

ARID2 suppressed the migration and invasion of HCC cells in vitro and inhibited the metastasis of HCC cells in vivo. (A) The protein levels of ARID2 in stably transfected HCC cells were examined by Western blot. (B and C) The effects of ARID2 on migration and invasion of HCC cells were investigated by Boyden chamber and transwell assay. (D and F) Gross morphology of livers injected with stably transfected HCC cells or control cells in left lobe and statistical analysis of foci number in injection lobe and noninjection lobes. Metastases on surface of injection lobe and noninjection lobes were counted under microscope. n_{(MHCC97-H-Vector)} and n_{(MHCC97-H-ARID2)}=7; n_{(PLC/PRF/5-SCR)}=10, n_{(PLC/PRF/5-shARID2)}=8. (E and G) Survival of the mice in intrahepatic metastasis mouse model injected with stably transfected HCC cells or control cells. MHCC97-H–ARID2 and control cells, n = 10, P = 0.0065; PLC/PRF/5-shARID2 and control cells, n = 7, P = 0.0029. (H and J) Fluorescence of metastases generated in distant seeding mouse model tail-vein-injected with stably transfected HCC cells or control cells was monitored, n = 5. (I and K) Survival of mice in the distant-seeding mouse model. MHCC97-H-ARID2 and control cells, n = 8, P = 0.022; PLC/PRF/5-shARID2 and control cells, n = 7, P = 0.0008. (L) Primary tumors and metastatic lesions of HCC mouse models. Mice of P53/Ras^G12D driven and DEN (25 mg/kg) induced HCC mouse models were euthanized at 4 and 13 months old, respectively. (M and N) Number of primary tumors (liver) and metastatic lesions (lung) of HCC mouse models. PK, n = 14; PKA, n = 13; Arid2^fl/fl and Arid2^fl/fl; AlbCre, n = 5. (O) Survival of PK and PKA mice. n = 10, P = 0.0095. Data were analyzed using Student’s t test. PK: P53^fl/fl; LSL-Ras^G12D; AlbCre. PKA: P53^fl/fl; LSL-Ras^G12D; Arid2^fl/fl; AlbCre. All *P < 0.05, **P < 0.01, ***P < 0.001, ns: not significant.