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. 2020 Feb 18;117(9):4770–4780. doi: 10.1073/pnas.1914937117

Fig. 5.

Fig. 5.

Inhibition of DNMT1 rescued the suppression of migration and invasion by ARID2 in HCC cells. (A and D) RT-qPCR was performed to investigate the mRNA levels of ARID2, DNMT1 and Snail of ARID2 overexpressing HCC cells and control cells treated with shRNA targeted DNMT1 or DNMT1 inhibitors 5-AZA (5 μM) and 6-TG (3 μM) for 48 h. (B and E) The alterations of Snail and DNMT1 protein level were examined by Western blot in ARID2-overexpressing HCC cells and control cells treated with shRNA targeted DNMT1 or DNMT1 inhibitors for 48 h. (C and F) Boyden chamber and transwell assay were employed to assess the migration and invasion of ARID2-overexpressing MHCC97-H and PVTT cells which DNMT1 was inhibited by shRNA or treated with DNMT1 inhibitors for 48 h. Data were analyzed using Student’s t test. All **P < 0.01, ***P < 0.001.