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. 2020 Feb 14;117(9):4653–4663. doi: 10.1073/pnas.1919409117

Fig. 4.

Fig. 4.

Nanobodies inhibited LIN28:pre-let-7:TUT4 complex assembly in vitro. (A) RNA EMSA of a titration of Nb-S2A4 with LIN28 (1.25 µM), NTUT282−759 (2 µM), and 32P-labeled pre-let-7g. Nb-S2A4 was added at 0.5, 1, 1.5, 2, 2.5, 3, 3.5, and 4 µM, respectively. *RNA alone; **RNA:LIN28 binary complex; ***RNA:LIN28:NTUT4 ternary complex. (B and C) RNA EMSA of LIN28 (1.25 µM), NTUT282−759 (2 µM) with 32P-labeled pre-let-7g, and nanobody inhibitors and complex binders. Nanobodies were added at 1.5-fold, 3.75-fold, and 7.5-fold molar excess to NTUT282−759. Nb.BV025 and Nb-R1A12 were used as negative controls. *RNA alone; **RNA:LIN28 binary complex; ***RNA:LIN28:NTUT4 ternary complex.