Fig. 7.

B cells support poststroke neurogenesis. (A) Brain Explorer surface rendering shows neurogenic regions with bilateral B cell diapedesis after stroke. (B) B cell depletion did not alter basal neurogenesis in the dentate gyrus in the absence of stroke. (C) Stroke induced neurogenesis (i.e., doublecortin [DCX]⁺ cells) in the ipsilesional (ipsi) hemisphere of wild type (WT; black circles) mice but not B cell-depleted (red squares) mice (D) independent of changes in granule cell layer (GCL) volume. (E) Stroke increased bromodeoxyuridine (BrdU)⁺ cells in the dentate gyrus over contralesional (contra) numbers only in WT mice, (F) as shown by immunohistochemical detection of BrdU-positive cells, that was lost with long-term B cell depletion of healthy mice. Significance determined by one-way ANOVA (*P < 0.05; **P < 0.01; ***P < 0.001 vs. ipsilesional hemisphere unless indicated by brackets).