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. 2020 Feb 18;117(9):4664–4674. doi: 10.1073/pnas.1915534117

Fig. 5.

Fig. 5.

Ubl-containing proteins together with the loosely folded protein casein activate proteasomal ATPases and thus replace the requirement for ubiquitin chains. (A) Rates of ATP hydrolysis as percent activity of 26S proteasomes alone (gray shaded bar). Rates of ATP hydrolysis upon addition of linear hexa-ubiquitin chains (6Ub) or Rad23B with (black bars) or without (white bars) casein. (B) Rates of ATP hydrolysis as percent of the activity of 26S proteasomes alone (gray shaded bar). Rates of ATP hydrolysis upon addition of 6Ub or addition of Ddi2 with (black bars) or without (white bars) casein. (C) Rates of ATP hydrolysis as percent activity of 26S proteasomes alone (gray shaded bar). Rates of ATP hydrolysis upon addition of 6Ub, wild-type Parkin (WT), or Parkin with the R42P mutation (R42P) with (black bars) or without (white bars) casein. Error bars are SEM (n = 3). *P < 0.05; **P < 0.01.