Table 3. Validation of qTGW2 using two NIL populations.

Population	Trait	Phenotype (Mean ± SD)		P	A	R2(%)
		NILTQ	NILIRBB52
HY2	TGW	25.23 ± 0.29	26.17 ± 0.34	<0.0001	0.47	60.94
	GL	8.081 ± 0.045	8.189 ± 0.049	<0.0001	0.054	48.26
	GW	2.784 ± 0.021	2.826 ± 0.025	<0.0001	0.021	36.67
	LWR	2.915 ± 0.024	2.911 ± 0.031	0.5690
HY3	TGW	22.65 ± 0.24	23.56 ± 0.26	<0.0001	0.45	66.02
	GL	7.919 ± 0.041	8.040 ± 0.036	<0.0001	0.061	62.12
	GW	2.652 ± 0.017	2.678 ± 0.018	<0.0001	0.013	22.74
	LWR	3.000 ± 0.022	3.017 ± 0.015	0.0003	0.009	12.94

Notes.

TGW

1,000-grain weight (g)

GL

Grain length (mm)

GW

Grain width (mm)

LWR

Length/width ratio

A

additive effect of replacing a Teqing allele with a IRBB52 allele

D

dominance effect

R2

proportion of the phenotypic variance explained by the QTL.

NIL^TQ

and NIL^IRBB52 Near-isogenic lines with Teqing and IRBB52 homozygous genotypes in the segregating region, respectively