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. 2020 Mar 3;12:1579–1585. doi: 10.2147/CMAR.S225489

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© 2020 Zhao et al.

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Overexpression of miR503HG led to altered miR-155 and caspase-3 expression. C33A and SiHa cells were transfected with a miR503HG expression vector. Overexpression of miR503HG was confirmed by qPCR at 24h post-transfection (A). qPCR results showed that miR503HG overexpression in CSCC cells led to the downregulation of miR-155 (B). In contrast, qPCR and Western blot experiments showed that miR503HG overexpression led to increased expression levels of caspase-3 at both mRNA (C) and protein (D) levels. Experiments were repeated 3 times and mean values were presented (*p<0.05).