Table 1.

Phytochemical analyses for M. radiotolerans MAMP 4754 crude extract.

Phytochemical test	Method	Observations	M. radiotolerans MAMP 4754 crude
Tannins	Add 2-3 drops of FeCl3 to 1 mL endophyte extract	Blackish-blue or blackish-green colour	−
Alkaloids	Add 2-3 drops of Dragendorff's reagent to 1 mL endophyte extract	Turbidity or precipitation formation	+
Flavonoids	Add 2-3 drops of NaOH to 1 mL endophyte extract	Yellow colour formation	+
Saponins	Add 2-3 drops of olive oil to 5 mL endophyte extract. Shake vigorously	Froth or foam formation	−
Steroids	Add 1 mL of CHCl3. Add 2-3 drops of conc. H2SO4 to 1 mL endophyte extract.	Reddish brown ring	+

The (+) and (−) represent the presence and absence of the phytochemicals.