Fig. 3.

Expression of miR-141 is down-regulated in eutopic and ectopic endometria and miR-141 overexpression inhibited the TGF-β1-induced EMT and invasion abilities of ISK cells in vitro. a miR-141 was detected among the three tissue samples by qRT-PCR analysis. b, c The TGF-β1, SMAD2, p-SMAD2, vimentin and E-cadherin protein expression levels were determined by western blot analysis in ISK cells transfected with the miR-141 mimic (2 µg/mL) or miR-141 shRNA (2 µg/mL) for 24 h. d, e Phenotypic analyses of cells transfected with the miR-141 mimic or miR-141 shRNA by transwell assay. The numbers of migrated cells were counted in the microscope fields. f, g ISK cells were transfected with miR-141 or miR-NC (2 µg/mL) and then treated with or without TGF-β1 (10 ng/mL) for 24 h. The protein expression levels of E-cadherin and vimentin were determined by western blot assay. h, i Phenotypic analyses of endometrial adenocarcinoma cells by transwell assay. The cells transfected with miR-141 or miR-NC were stimulated with TGF-β1 (10 ng/mL) for 24 h. The numbers of migrated cells were then counted in the microscope fields. *P < 0.05, **P < 0.01 and NS non-significant